Midwives should provide reliable and valid information on influenza vaccination during pregnancy, considering they work directly with pregnant women, who are in the risk group, and in the priority group. Whereas in some countries it is routinely recommended to offer a seasonal flu vaccine to all pregnant women, in Croatia this is not the case. The goals of this research are to find out how much midwives know about influenza vaccination during pregnancy, whether they provide pregnant women with information, and whether there is a link between their knowledge and sociodemographic characteristics. The research involves 82 midwives of all levels of education in Croatia. A cross-sectional study was conducted using an online questionnaire. The overall knowledge of midwives on influenza vaccination during pregnancy is limited. 43% of respondents think that influenza vaccination during pregnancy is efficient, and 28% of them claim that it is safe. 30% of respondents know that pregnant women can get the inactivated vaccine, and only 21% of respondents know that they can get it in any trimester. 93% of midwives involved with the research claimed they don't provide pregnant women any information on influenza vaccination during pregnancy. The results show that there is a difference according to sociodemographic characteristics of respondents such as gender, level of education, years of service, vaccination of participants against influenza during the 2020/2021 season. Midwives play an important role in preventing complications of influenza by informing pregnant women on the importance of influenza vaccination. To fulfill this important role, midwives themselves need to be educated on influenza vaccination during pregnancy and provide pregnant women with information in their everyday work.