INTRODUCTION: Instrument nurses have multiple roles in the operating room before, during, and after surgery, and it includes maintaining sterile conditions during surgery, preparing surgical instruments, and assisting the surgeon during surgery. For a nurse to perform her task in the operating room, she must know the entire course of the operation, differentiate surgical instruments, and be prepared for quick and adequate reactions, since operating rooms are often unstable environments where the patient's condition can change from minute to minute. OBJECTIVE: The aim of the research was to investigate and present the opinion of nursing students at the Undergraduate Professional Study of Nursing on the role of an instrument nurse in the operating room. The specific objectives of the research are to present the opinion of nursing students on teamwork and relations within a multidisciplinary team in the operating room and the opinion on the level of education required to work in it. METHODS: Data for the research were collected through an online survey questionnaire designed by the author for the purposes of writing the final paper. The first part of the questionnaire collected socio-demographic data of respondents, and the second part of respondents' opinions on the role of instrumental nurse in the operating room, teamwork, and the level of education of nurses / technicians required to work in the operating room. Prior to completing the questionnaire, all respondents were provided with an informed consent form. The results were processed by descriptive statistics methods. RESULTS: A total of 53 subjects participated in the study, of which only 2 subjects were not in the operating room during the procedure. 100% of respondents believe that the role of instrumental nurse is important, 98.1% of respondents believe that teamwork is important in the operating room, but 56.6% of respondents believe that the role of instrumental nurse is neglected by other team members and 71.7% the respondent believes that there is a conflict within the team. 54.7% of respondents believe that during the studies they did not acquire enough clinical practice to work in the operating room and that the same requires additional education, which 98.1% of respondents believe. CONCLUSION: Nursing students believe that the instrument nurse has a significant role in surgical interventions, but the perception of the role of the instrument nurse by students, ward nurses and other health professionals is insufficiently researched