SUMMARY Introduction: As a result of rapid economic growth, interest in well-being and quality of life has increased, and the workplace is recognized as a place that enables individual satisfaction and growth, not just serving the environment in which economic goals are achieved. The quality of the working life of nurses is defined as the level of subjective satisfaction at the workplace with the achievement of organizational goals. The quality of working life is also the ability of employees to satisfy their personal needs by working in the organization and increase the quality of working life. Objective: The main objective of this research is to examine the quality of the working life of nurses in the Republic of Croatia. Methods: The research was conducted on a convenient sample of 348 nurses and technicians from the entire Republic of Croatia. The Brooks questionnaire on the quality of nurses' working life was used to examine the quality of nurses' working life. Results: Gender was not a significant predictor in the overall quality of working life. Female respondents achieve a statistically significantly lower result in the domain of work and family harmony. Respondents between the ages of 36 and 45 achieve a statistically significantly higher score in the domain of work-family harmony. Respondents who are married and have three or more children achieve statistically significantly the highest results in the overall quality of working life and in the domain of work-family harmony. Professional qualification, length of work experience and level of education were not significant predictors in the overall quality of working life. In the domain of work-family harmony, respondents who work in intensive care units achieve a statistically significantly lowest result. Shift and on-call work and longer overtime work are significant predictors of lower overall quality of work life and quality in the domains of work-family harmony, work organization and work content. Conclusion: Assessing the strengths and weaknesses of the work environment can be of great help when designing strategies to improve the quality of work life of healthcare professionals.