Intrahospital infections (nosocomial infections, hospital infections) are infections that occurred in the first 48 hours after the patient's admission to the hospital. Intrahospital infections are not present and the patient was not in contact with them until his arrival at the health institution. Because of their rapid expansion and resistance to certain drugs, intrahospital infections cause serious health problems. They also cause concern for the safety of patients, healthcare professionals, the patient's family and ultimately the entire community. Intrahospital infections cause increased mortality, morbidity and prolong the hospital stay, which leads to additional costs in the treatment of the patient. The aim of this research is to examine the knowledge of nurses/technicians about the prevention of intrahospital infections. A total of 100 respondents, mostly female, aged 30-49, with a secondary vocational education, participated voluntarily. The research was conducted through an online questionnaire published on a Facebook page that gathers nurses and technicians throughout the Republic of Croatia. This research examined the overall average knowledge of nurses, as well as the knowledge of nurses depending on the level of education and length of service. The obtained results indicate that the knowledge of nurses/technicians about preventing the spread of intrahospital infections is high (>80%), that there is a difference in knowledge depending on the level of education (nurses/technicians with a high degree of education have more knowledge than nurses/technicians with a completed secondary and higher education), and that there is no difference in knowledge regarding the length of work experience (respondents with longer work experience do not show statistically significantly greater knowledge than respondents with shorter work experience). In conclusion, nurses/technicians have a big, perhaps the main role in the prevention of the spread of intrahospital infections, for this reason it is necessary to regularly provide nurses/technicians with education in this area so that they can perform their work as well as possible and prevent the spread of infections in time.