According to research from 2020, breast cancer was the most common cause of cancer in women in Croatia and represents a public health problem. The etiology of the disease itself is complex, and the disease is influenced by various risk factors. Some of the risk factors of breast cancer can be prevented, such as: increased body weight, alcohol consumption, etc. We can conclude that prevention plays a significant role in this type of disease, therefore the aim of this paper was to investigate the knowledge of third-year undergraduate students of nursing, midwifery, radiological technology and physiotherapy about breast cancer, its risk factors, methods of diagnosis and prevention, and to determine whether there is a difference in knowledge of nursing students compared to students of midwifery, radiological technology and physiotherapy. The study was carried out using an online survey, with the participation of 100 individuals who responded to the questionnaire. Based on the results obtained according to the categorization of the level of knowledge about breast cancer, no respondent is considered to have no knowledge about breast cancer, 43% of respondents have a sufficient level of knowledge about breast cancer, and 57% have a high level of knowledge about breast cancer. Upon conducting a comparative analysis of the knowledge levels among students from various disciplines, a substantial and statistically significant distinction emerged between nursing students and those studying physiotherapy. Notably, nursing students exhibited a significantly greater depth of knowledge compared to their counterparts in physiotherapy, highlighting the discernible discrepancy between the two groups.