This paper focuses on thrombolytic therapy as one of the methods of acute ischemic stroke (AIS) treatment. Its goal is to achieve blood vessel recanalization, revascularising brain parenchyma affected by ischemia and thereby achieving more favourable functional outcomes. AIM OF THE RESEARCH: (1) To examine intravenous thrombolysis effects with regard to patients' gender. (2) To examine the effect of intravenous thrombolysis on treatment outcome (symptoms improvement, symptoms worsening, death). MATERIALS AND METHODS: The study included 100 patients (50 males, 50 females) with a diagnosis of AIMU, who were admitted to Emergency Department of the Karlovac General Hospital and treated with intravenous alteplase in the period from January 1, 2020 to January 1, 2023. The research is a restrospective cohort analysis and the sample is non-probabilistic (convenience sampling). RESULTS: No statistically significant difference was found between male and female patients after intravenous thrombolysis in any of the NIHSS segments (level of consciousness P=0,579; gaze/visual P=0,297; facial palsy P=0,674; left arm motor P=0,621; right arm motor P=0,651; left leg motor P=0,149; right leg motor P=0,407; language P>0,999; sensory P=0,674). The most common outcomes were symptoms improvement (52%); symptoms worsening (42%) and death (6%). The most significant statistical difference was observed in facial palsy (χ2=4,57; P=0,033) and sensory (χ2=3,85; P=0,049) symptoms improvment. There was no statistically significant differences in other segments of the NIHSS scores before and after intravenous thrombolysis. CONCLUSION: There was no statistically significant difference in the effects of intravenous thrombolysis between male and female patients. The most common outcome was improvement of symptoms, followed by worsening of symptoms, and the rarest outcome was death, which confirms the effectiveness of AIS treatment with intravenous thrombolysis.