OBJECTIVE OF THE RESEARCH: The research presented in this paper was to investigate the presence and intensity of professional stress and burnout syndrome among employees of the oncology departments of the General Hospital (OB) Karlovac and the Tumor Clinic (KZT) Zagreb. Also, factors related to the burnout syndrome of healthcare workers at the workplaces were investigated. MATERIALS AND METHODS: 118 respondents participated, who filled out a survey questionnaire at the oncology departments of OB Karlovac and KZT Zagreb. The research was conducted as an observational and cross-sectional study on a convenient sample. RESULTS: A higher level of stress among the employees of OB Karlovac and KZT Zagreb was shown by respondents from Ob Karlovac (t=2.09; P=0.039). Also, the level of burnout at work is statistically significant among respondents Ob Karlovac (t=2.09; P=0.039). No positive correlation was found between gender, age, level of education, as opposed to the length of work experience, on the total experience of stress and the incidence of burnout syndrome. An increase in length of service by a year leads to an expected increase in burnout at work by an average of 2.79. The parameter is statistically significant (t=2.04; P=0.022). It was found that a statistically significant majority of medical personnel do not take anxiolytics to help them perform their duties normally (χ2=51.56; df=1; P<0.001) CONCLUSION: Respondents from the oncology departments of OB Karlovac show a higher presence of professional stress and burnout syndrome than their colleagues at KZT Zagreb. The influence of sociodemographic characteristics such as gender, age, and level of education is not related to the overall experience of stress and the occurrence of burnout syndrome, except for the length of work experience. Respondents do not use anxiolytics as an aid in performing their duties, but the help of a psychologist in their team is welcome. They describe satisfaction with their life with a high rating.