prikaz prve stranice dokumenta Protective role of strawberry tree ( Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro
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Scientific paper - Original scientific paper
Protective role of strawberry tree ( Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro
Journal of Apicultural Research, x (2022); 1-10. https://doi.org/10.1080/00218839.2022.2047421
Jurič, Andreja; Brčić Karačonji, Irena; Žunec, Suzana; Katić, Anja; Gašić, Uroš; Milojković Opsenica, Dušanka; Kopjar, Nevenka

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Jurič, A., Brčić Karačonji, I., Žunec, S., Katić, A., Gašić, U., Milojković Opsenica, D. & Kopjar, N. (2022). Protective role of strawberry tree ( Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro. Journal of Apicultural Research, x., 1-10. doi: 10.1080/00218839.2022.2047421

Jurič, Andreja, et al. "Protective role of strawberry tree ( Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro." Journal of Apicultural Research, vol. x, 2022, pp. 1-10. https://doi.org/10.1080/00218839.2022.2047421

Jurič, Andreja, Irena Brčić Karačonji, Suzana Žunec, Anja Katić, Uroš Gašić, Dušanka Milojković Opsenica and Nevenka Kopjar. "Protective role of strawberry tree ( Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro." Journal of Apicultural Research x (2022): 1-10. https://doi.org/10.1080/00218839.2022.2047421

Jurič, A., et al. (2022) 'Protective role of strawberry tree ( Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro', Journal of Apicultural Research, x, pp. 1-10. doi: 10.1080/00218839.2022.2047421

Jurič A, Brčić Karačonji I, Žunec S, Katić A, Gašić U, Milojković Opsenica D, and sur.. Protective role of strawberry tree ( Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro. Journal of Apicultural Research [Internet]. 2022 March 22 [cited 2024 May 05];x:1-10. doi: 10.1080/00218839.2022.2047421

A. Jurič, et al., "Protective role of strawberry tree ( Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro", Journal of Apicultural Research, vol. x, pp. 1-10, March 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:234858. [Accessed: 05 May 2024]

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Metadata
Title (english)Protective role of strawberry tree ( Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro
AuthorAndreja Jurič
AuthorIrena Brčić Karačonji
AuthorSuzana Žunec
AuthorAnja Katić
AuthorUroš Gašić
AuthorDušanka Milojković Opsenica
AuthorNevenka Kopjar
Author's institutionUniversity of Rijeka
Faculty of Health Studies
(Department of Basic Medical Sciences)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Strawberry tree (Arbutus unedo L.) honey (STH) is widely used as part of traditional medicine in the Mediterranean area, especially in the treatment of wounds, burns and infections. In this study, we investigated the cyto-/genoprotective properties of STH at cell level following short-term exposure to UVB radiation at 2 kJ/m2. Isolated human peripheral blood lymphocytes (PBL) were selected as model cells. Phenolics in STH were determined using an ultra-high-performance liquid chromatograph (UHPLC) coupled to a linear ion trap-Orbitrap hybrid mass spectrometer (LTQ Orbitrap MS), while homogentisic acid (HGA) was quantified using a gas chromatograph-mass spectrometer (GC-MS). Primary DNA damage in PBLs treated with STH before and after irradiation was assessed by alkaline comet assay, while their viability was determined using the apoptosis/necrosis assay. The levels of thiobarbituric acid reactive substances (TBARS) and glutathione (GSH) were determined in plasma samples. STH applied as pre-treatment at a concentration equivalent to its average daily portion efficiently counteracted the cytotoxic and genotoxic effects and diminished UVB-induced oxidative stress. Post-radiation treatment with STH was not as beneficial. Protective effects could be associated to the complex phytochemical profile of STH and the high content of HGA (306.8 mg/kg). It was confirmed that an intake of a complex mixture of bioactive constituents as STH prior to potential cyto/genotoxic insults stimulated a broad-spectrum of protective mechanisms, including intracellular antioxidative defence and DNA repair systems that helped pre-treated cells to counteract harmful exposures more efficiently.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleJournal of Apicultural Research
Numberingvol. x, pp. 1-10
p-ISSN0021-8839
e-ISSN2078-6913
DOIhttps://doi.org/10.1080/00218839.2022.2047421
URN:NBNurn:nbn:hr:184:234858
Publication2022-03-22
Document URLhttps://www.tandfonline.com/doi/full/10.1080/00218839.2022.2047421
Type of resourceText
Access conditionsClosed access
Terms of useLicence In copyright icon
Created on2024-01-15 13:51:22