This work will present the anatomy and physiology of the large and small intestines, the definitions of colostomy and ileostomy, the complications associated with colostomy and ileostomy, the aids and procedures for ostomy care, the healthcare of patients with a stoma, nutrition, the role of the enterostomal therapist, and the importance of preoperative marking of the stoma site. The aim of this research is to examine and compare the knowledge of part-time undergraduate students and part-time graduate students at the Faculty of Health Studies in Rijeka, who are employed in different work environments and have varying years of work experience, regarding the elimination stomas of the digestive system. The research will be conducted through an online survey, which will be completed by part-time students in the 2nd and 3rd years of the undergraduate professional study of Nursing, and part-time university graduate students in Management in Nursing and Promotion and Protection of Mental Health. The final results of the research on the general knowledge of students about the elimination stomas of the digestive system will also be presented. This is significant because many nurses/technicians at their work sites may not have encountered stomas or provided health care for people with stomas, or they may have encountered them frequently but are still not sufficiently familiar with the procedures for stoma care. As the number of people with stomas increases every day, the need for training nurses and technicians becomes greater. Quality health care for people with a stoma is very important during the preoperative, intraoperative, and postoperative periods. Additionally, continuous health care and education of the patient after hospitalization are crucial for physical and mental health and for their earliest return to a normal life routine. In the research part of the paper, the data obtained through a survey on the knowledge of part-time undergraduate and part-time graduate nursing students about the elimination stomas of the digestive system, considering their workplace and the number of years of work experience, were processed and analyzed.