Gynecological screening is part of women's health care. Due to the way in which the examination is carried out, it is perceived by women as emotional and intimate. How exactly women perceive a gynecological examination has not been adequately studied, but some studies have shown that the experience is negative. Sometimes women postpone gynecological examinations due to previous negative experiences, which can lead to late detection of various pathological conditions. The aim of this study was to investigate how women experience a gynecological screening in relation to their age, level of education, age of first gynecological examination and the degree of social support when participating in the examination. One of the objectives was to investigate which emotions respondents experience most frequently when they go for a gynecological screening and who their social support for attending the screening is. The research was conducted using an online questionnaire developed specifically for this study. 140 respondents from different parts of the Republic of Croatia participated. The study showed that older age, higher education level and older age at first gynecological examination were not associated with a positive experience with gynecological screening, but a higher level of social support was. The results show that adequate social support is very important for women to go for a gynecological screening, with the most common source of social support being the mother. During a gynecological screening, negative emotions such as discomfort or shame occur more frequently, while positive ones such as satisfaction and relief occur less frequently. Adequate communication, education and appropriate health equipment contribute to a positive experience of a gynecological screening. It is therefore very important to provide each patient with individualized care with empathy and understanding.