Aim: To compare information literacy - characteristics and habits of online databases usage among students of different graduate and undergraduate studies of physiotherapy in Croatia. Methods: The research was conducted by using an online-shared survey questionnaire on the sample of students from different graduate and undergraduate studies in Croatia. The questionnaire on characteristics and habits of online databases usage consisted of three parts. The first part was meant to gather demographic data, the second covered questions about information related to characteristics of online database searching, and the third part consisted of questions about its frequency. Results: There were 395 respondents (31% response rate) who took part in this study. Most of them were female (n=316; 80%). The youngest age of the respondents was 18, the oldest 53, and the mean age was median 22 years. The most used database was PubMed (99,5%), followed by Google Scholar (59,7%), Pedro (56,2%), Web of Science (37,5%) and Cochrane (24,1%). When comparing students based on their academic institution, it was found that there are statistically significant differences in frequency of online database usage for the purpose of fulfilling the academic duties (p<0,001), in the sense of higher frequency of usage among Veleučilišt „Lavoslav Ružička“ u Vukovaru students compared to others. The same usage habits do not differ significantly in regard to the academic institution when observed independently of fulfilling academic duties. Comparing students based on whether they are graduate or undergraduate, it was shown that graduate students use online databases considerably more often than undergraduate students for fulfilling academic duties (p<0,001), as well as for unassociated purposes (p<0,001). Conclusion: There are differences in characteristics and habits of online databases usage among students of different graduate and undergraduate studies of physiotherapy in Croatia. Veleučilište „Lavoslav Ružička“ u Vukovaru students use online databases more often than students of other academic institutions, just as graduate students use them more often than undergraduate students.