In today's age, malignant diseases are an increasingly widespread phenomenon all over the world, they affect younger and younger age groups and are a major global public health problem. In the Republic of Croatia, there are national preventive programs for the early detection of breast, cervical and colon cancer. The goal of these programs is to detect malignant disease in the early stages, which can influence the reduction of the mortality rate of the population. The aim of the paper is to investigate and show the response rate to the National preventive programs for the early detection of colon cancer and breast cancer among health workers in the Department of Emergency Medicine of Karlovac County and the Karlovac Health Center. The specific goals of the research are to examine the rate of response to National Preventive Programs by gender, to analyze according to the level of education of healthcare workers, and to examine the most common reasons for non-response. Material: An anonymous survey was conducted with the approval of the ethical commissions of the institutions. The research was conducted among 52 health workers of both sexes of the above-mentioned institutions. Method: Hypotheses testing with collected variables, chi-square test and difference of proportions test were used. Results: Response to mammography was 86.21% of health workers, and 75% responded to the test for occult bleeding, which is less than 90% of health workers. In this sample, it is evident that the response to acute bleeding is higher in men, while the expectation was a higher response in women. With regard to vocational education, there is no statistically significant difference in the response of Secondary education and university degree. None of the stated reasons for not going to preventive examinations was "I have no problems", so that hypothesis had to be rejected. Conclusion: By analyzing the sample, all the mentioned hypotheses were rejected. Regardless of the frequency and the increasingly common occurrence among younger people, preventive programs are still not popular. It should be taken into account that this is a small sample and that research should be done within other health institutions on a larger number of respondents in order to get a broader picture.