Introduction: Working in an outhospital emergency medical service is extremely demanding and requires great physical and mental effort. During their work assignments, emergency workers encounter emergencies, life-threatening lives and the need to make quick and adequate decisions on a daily basis to help provide assistance to those in need. The level of stress in emergency workers is at a high level every day. The work of a dispatcher in a medical reporting unit is accompanied by a high level of stress and a high degree of responsibility, requires composure and work under great daily pressure and must balance between many tasks at the same time and make quick and effective decisions. Aim of the study: The aim of the study is to investigate the influence of stress factors and the overall experience of stress and the perception of stress in employees in ZZHM depending on whether they work as a dispatcher or as a nurse / technician in team 1 or team 2. Methods: The research was conducted on a sample of 92 respondents employed in the Institute of Emergency Medicine of the Republic of Croatia. The questionnaire "Questionnaire on stressors in the workplace of hospital health workers" was used to examine stress, and the "Perceived Stress Scale" was used to examine stress perception. Results: Workplace organization and financial issues is the stress factor that causes the highest level of stress in respondents. Dispatchers in relation to nurses / technicians in Team 1 and Team 2 experience more stressful workplace organization and financial issues, public criticism and lawsuits and shift work. The overall experience of stress in dispatchers is statistically more significant compared to Team 1 and Team 2 employees. Conclusion: Medical staff employed in the emergency department are exposed to a far greater amount of stress than others. Accordingly, a way needs to be found to improve working conditions and ensure a sufficient number of staff to reduce the workload. It is also necessary to devise strategies through which to detect the occurrence of stress among emergency workers in a timely manner and provide them with the need and appropriate assistance.