prikaz prve stranice dokumenta Cytotoxic activity of strawberry tree (Arbutus unedo L.) honey, its extract, and homogentisic acid on CAL 27, HepG2, and Caco-2 cell lines
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Scientific paper - Original scientific paper
Cytotoxic activity of strawberry tree (Arbutus unedo L.) honey, its extract, and homogentisic acid on CAL 27, HepG2, and Caco-2 cell lines
Arhiv za higijenu rada i toksikologiju, 73 (2022), 2; 158-168. https://doi.org/10.2478/aiht-2022-73-3653
Jurič, Andreja; Huđek Turković, Ana; Brčić Karačonji, Irena; Prđun, Saša; Bubalo, Dragan; Durgo, Ksenija

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Jurič, A., Huđek Turković, A., Brčić Karačonji, I., Prđun, S., Bubalo, D. & Durgo, K. (2022). Cytotoxic activity of strawberry tree (Arbutus unedo L.) honey, its extract, and homogentisic acid on CAL 27, HepG2, and Caco-2 cell lines. Arhiv za higijenu rada i toksikologiju, 73. (2), 158-168. doi: 10.2478/aiht-2022-73-3653

Jurič, Andreja, et al. "Cytotoxic activity of strawberry tree (Arbutus unedo L.) honey, its extract, and homogentisic acid on CAL 27, HepG2, and Caco-2 cell lines." Arhiv za higijenu rada i toksikologiju, vol. 73, no. 2, 2022, pp. 158-168. https://doi.org/10.2478/aiht-2022-73-3653

Jurič, Andreja, Ana Huđek Turković, Irena Brčić Karačonji, Saša Prđun, Dragan Bubalo and Ksenija Durgo. "Cytotoxic activity of strawberry tree (Arbutus unedo L.) honey, its extract, and homogentisic acid on CAL 27, HepG2, and Caco-2 cell lines." Arhiv za higijenu rada i toksikologiju 73, no. 2 (2022): 158-168. https://doi.org/10.2478/aiht-2022-73-3653

Jurič, A., et al. (2022) 'Cytotoxic activity of strawberry tree (Arbutus unedo L.) honey, its extract, and homogentisic acid on CAL 27, HepG2, and Caco-2 cell lines', Arhiv za higijenu rada i toksikologiju, 73(2), pp. 158-168. doi: 10.2478/aiht-2022-73-3653

Jurič A, Huđek Turković A, Brčić Karačonji I, Prđun S, Bubalo D, Durgo K. Cytotoxic activity of strawberry tree (Arbutus unedo L.) honey, its extract, and homogentisic acid on CAL 27, HepG2, and Caco-2 cell lines. Arhiv za higijenu rada i toksikologiju [Internet]. 2022 [cited 2024 November 11];73(2):158-168. doi: 10.2478/aiht-2022-73-3653

A. Jurič, A. Huđek Turković, I. Brčić Karačonji, S. Prđun, D. Bubalo and K. Durgo, "Cytotoxic activity of strawberry tree (Arbutus unedo L.) honey, its extract, and homogentisic acid on CAL 27, HepG2, and Caco-2 cell lines", Arhiv za higijenu rada i toksikologiju, vol. 73, no. 2, pp. 158-168, 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:102909. [Accessed: 11 November 2024]

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Title (english)Cytotoxic activity of strawberry tree (Arbutus unedo L.) honey, its extract, and homogentisic acid on CAL 27, HepG2, and Caco-2 cell lines
Title (croatian)Citotoksično djelovanje meda obične planike (Arbutus unedo L.), ekstrakta i homogentizinske kiseline na stanične linije CAL 27, HepG2 i Caco-2
AuthorAndreja Jurič https://orcid.org/0000-0002-9279-5159
AuthorAna Huđek Turković
AuthorIrena Brčić Karačonji
AuthorSaša Prđun
AuthorDragan Bubalo
AuthorKsenija Durgo
Author's institutionUniversity of Rijeka
Faculty of Health Studies
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Public Health and Health Care
Abstract (english)
Strawberry tree (Arbutus unedo L.) honey (STH), also known as “bitter honey”, is a traditional medicine widely used in the Mediterranean area. Regardless of geographical origin, it usually has a very high content of phenolic compounds and strong antioxidant capacity. Yet, little is still known about the effects of STH, its phenolic extract (STHE), and its main bioactive compound – homogentisic acid (HGA) – at the cell level. The aim of this study was to estimate total phenolic content, DPPH radical scavenging activity, and ferric reducing antioxidant power of STH made in Croatia and investigate cytotoxic and pro-oxidative effects of STH, STHE and HGA on three human cell lines: tongue squamous cell carcinoma (CAL 27), hepatocellular carcinoma (HepG2), and epithelial colorectal adenocarcinoma cells (Caco-2) cells. These substances were tested at four concentrations (0.5–5× average human daily intake of STH) and over 30 min and 1 and 2 h. Croatian STH had a total phenolic content of 1.67 g gallic acid equivalents (GAE) per kg of honey, DPPH radical scavenging activity of 2.96 mmol Trolox equivalents (TE) per kg of honey, and ferric reducing antioxidant power (FRAP) of 13.5 mmol Fe2+ per kg of honey. Our results show no clear and consistent time- or concentration-dependent cytotoxicity in any of the cell lines. ROS levels in all the three cell types at almost all exposure times were not significantly higher than control. The most important observation is that the tested substances have low cytotoxicity and high biocompatibility, regardless of concentration, which is a good starting point for further research of their biological effects in other models.
Abstract (croatian)
Med obične planike (Arbutus unedo L.) (STH), poznat kao “gorki med”, tradicionalno se koristi u narodnoj medicini na sredozemnom području. Bez obzira na zemljopisno podrijetlo, obično ima vrlo visok udio fenolnih spojeva i snažan antioksidacijski kapacitet. Ipak, još uvijek se malo zna o učincima STH-a, njegova ekstrakta (STHE), kao i dominantnoga fenolnog spoja – homogentizinske kiseline (HGA) – na staničnoj razini. Cilj ovoga istraživanja bio je utvrditi ukupni sadržaj fenola, antioksidacijski kapacitet metodom DPPH i FRAP u STH-u, proizvedenome u Hrvatskoj, te ispitati citotoksične i prooksidacijske učinke STH-a, STHE-a i HGA-e na tri ljudske stanične linije: karcinoma pločastih stanica jezika (CAL 27), hepatocelularnoga karcinoma jetre (HepG2) i adenokarcinoma epitela debelog crijeva (Caco-2). STH, STHE i HGA ispitani su u četirima koncentracijama (0,5–5× prosječni dnevni unos STH-a u ljudi) i tijekom 30 minuta te tijekom jednog i dva sata. Hrvatski STH imao je visok ukupan sadržaj fenola (1,67 g ekvivalenata galne kiseline po kg meda i snažan antioksidacijski kapacitet (2,96 mmol Trolox ekvivalenata po kg meda i 13,5 mmol Fe2+ po kg meda). Dobiveni rezultati ne pokazuju jasnu i dosljednu citotoksičnost, ovisno o vremenu ili koncentraciji, ni u jednoj staničnoj liniji. Razine reaktivnih kisikovih vrsta u svim trima tipovima stanica u gotovo svim vremenima izlaganja nisu bile značajno veće od kontrole. Najvažnije je zapažanje da ispitivane tvari imaju nisku citotoksičnost i visoku biokompatibilnost, bez obzira na koncentraciju, što je dobra polazna točka za daljnja istraživanja njihovih bioloških učinaka na drugim modelima.
Keywords (english)
Keywords (croatian)
Languageenglish
Languagecroatian
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleArhiv za higijenu rada i toksikologiju
Numberingvol. 73, no. 2, pp. 158-168
p-ISSN0004-1254
e-ISSN1848-6312
DOIhttps://doi.org/10.2478/aiht-2022-73-3653
URN:NBNurn:nbn:hr:184:102909
Publication2022
Document URLhttps://hrcak.srce.hr/279733
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-01-15 10:03:29