Introduction: During a long period of physical effort, if the body does not have the ability to recover energy resources, it is defended by various mechanisms, most often with fatigue. When it comes to top athletes, fatigue is usually neglected or overlooked, given the assumption that athletes who are in daily training cannot be tired. Fatigue is defined as a condition of an organism characterized by a reduction in work performance, i.e., abilities resulting from a certain long-lasting and hard work. Aim: The aim of the study is to identify the difference in fatigue in female athletes and control groups and to establish the correlation between total tiredness and physical and psychosocial health. Participants and methods: In this study there were 22 girls from the school age of 8-14 years who regularly train handball and 20 girls of the same age who are not engaged in sports on a regular basis three times a week. The girls enrolled in the study underwent a study through the quality of life survey related to fatigue (PedsQL- Fatigue) and general quality of life (PedsQL-General Health 4.0). Through the general health survey, physical and psychosocial health was examined for the purpose of correlation with fatigue. Their parents have given written consent that their children may participate in the study in order to create this study. Results: The results showed that the average value of general fatigue is higher in female athletes than without a statistically significant difference. Also, sleep-related fatigue, cognitive fatigue, and overall fatigue showed higher value in female athletes than with statistically significant differences. It also identified a statistically significant correlation between total fatigue and psychosocial and physical health in female athletes, while the control group did not establish a connection. Conclusion: The investigation revealed the existence of greater fatigue in female athletes compared to control group, but without statistical significance. Thus the initial hypothesis is not confirmed. It also identified a statistically significant correlation between total fatigue and psychosocial and physical health in female athletes, while the control group did not establish a connection. Thus, the sport affects fatigue in a way that increases it in children of school age.