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undergraduate thesis
POLIMORFIZMI GENA ZA ČIMBENIK NEKROZE TUMORA ALFA U ŽENA SA SPONTANIM PRIJEVREMENIM PORODOM
2018. urn:nbn:hr:184:722644
Mance, Katarina
University of Rijeka
Faculty of Health Studies
Department of Laboratory and Radiological Diagnostics

Cite this document

Mance, K. (2018). POLIMORFIZMI GENA ZA ČIMBENIK NEKROZE TUMORA ALFA U ŽENA SA SPONTANIM PRIJEVREMENIM PORODOM (Undergraduate thesis). Rijeka: University of Rijeka, Faculty of Health Studies. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:722644

Mance, Katarina. "POLIMORFIZMI GENA ZA ČIMBENIK NEKROZE TUMORA ALFA U ŽENA SA SPONTANIM PRIJEVREMENIM PORODOM." Undergraduate thesis, University of Rijeka, Faculty of Health Studies, 2018. https://urn.nsk.hr/urn:nbn:hr:184:722644

Mance, Katarina. "POLIMORFIZMI GENA ZA ČIMBENIK NEKROZE TUMORA ALFA U ŽENA SA SPONTANIM PRIJEVREMENIM PORODOM." Undergraduate thesis, University of Rijeka, Faculty of Health Studies, 2018. https://urn.nsk.hr/urn:nbn:hr:184:722644

Mance, K. (2018). 'POLIMORFIZMI GENA ZA ČIMBENIK NEKROZE TUMORA ALFA U ŽENA SA SPONTANIM PRIJEVREMENIM PORODOM', Undergraduate thesis, University of Rijeka, Faculty of Health Studies, accessed 20 November 2024, https://urn.nsk.hr/urn:nbn:hr:184:722644

Mance K. POLIMORFIZMI GENA ZA ČIMBENIK NEKROZE TUMORA ALFA U ŽENA SA SPONTANIM PRIJEVREMENIM PORODOM [Undergraduate thesis]. Rijeka: University of Rijeka, Faculty of Health Studies; 2018 [cited 2024 November 20] Available at: https://urn.nsk.hr/urn:nbn:hr:184:722644

K. Mance, "POLIMORFIZMI GENA ZA ČIMBENIK NEKROZE TUMORA ALFA U ŽENA SA SPONTANIM PRIJEVREMENIM PORODOM", Undergraduate thesis, University of Rijeka, Faculty of Health Studies, Rijeka, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:184:722644

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Metadata
TitlePOLIMORFIZMI GENA ZA ČIMBENIK NEKROZE TUMORA ALFA U ŽENA SA SPONTANIM PRIJEVREMENIM PORODOM
Title (english)TUMOR NECROSIS FACTOR ALFA POLYMORPHISMS IN WOMEN WITH SPONTANEOUS PREMATURE BIRTH
AuthorKatarina Mance
MentorNina Pereza (mentor)
Committee memberSaša Ostojić (predsjednik povjerenstva)
Committee memberSmiljana Ristić (član povjerenstva)
Committee memberNina Pereza (član povjerenstva)
GranterUniversity of Rijeka
Faculty of Health Studies
(Department of Laboratory and Radiological Diagnostics)
Rijeka
Defense date and country2018-09-12, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Human Genetics, Genomics and Proteomics
Abstract
Cilj: Čimbenik nekroze tumora alfa (engl. Tumor Necrosis Factor Alfa, TNF-alfa) kao ključni proupalni citokin, koji ima ulogu u regulaciji stanične diferencijacije, proliferacije i smrti, te kod upale, urođenih i stečenih imunoloških odgovora, važan je u regulaciji poroda tako što sudjeluje u remodeliranju cerviksa i fetalnih membrana promicanjem produkcije degradacije kolagena. U našem istraživanju istraživali smo genetičku povezanost između polimorfizama gena za TNF-alfu u žena i podložnosti za spontani prijevremeni porod (SPP) te smo ispitivali njihov utjecaj na klinička obilježja žena sa SPP-om u slovenske i hrvatske populacije. Ispitanici i metode: Istraživanje je provedeno kao istraživanje parova (engl. case-contol study) u kojem je sudjelovalo 138 žena koje su imale spontani prijevremeni porod prije 37.tjedna trudnoće i 139 žena sa terminskim porodom koje spadaju u kontrolnu skupinu. Kao metode za identifikaciju polimorfizama gena TNF-alfa (-238 G/A ili rs361525 i -308 G/A ili rs1800629) koristile su se: lančana reakcija polimeraze, polimorfizam duljine restrikcijskih te gel elektrozoreza. Rezultati: Učestalosti genotipova i alela su se statistički značajno razlikovale kod žena sa SPP-om i kontrolne skupine kod polimorfizma TNF-alfa -238. Kod žena sa SPP-om u usporedbi s kontrolnom skupinom, učestaliji su genotipovi AG (X2=7,32; P=0,025) i alel A (X2=7,48; P=0,006). Izgled za SPP je 3, 20 puta veći (95% CI= 1,30 – 7,83, P=0,011) kod žena s genotipovima AA i AG nego kod žena s genotipom GG. Razlike u učestalosti genotipova u polimorfizmu TNF-alfa -308 između skupina ispitanica nisu statistički značajne te ni kod povezanosti istog s izgledima za SPP prema genitičkim modelima nije nađena statistički značajna vrijednost. Nije pronađen utjecaj niti jednog polimorfizma na klinička obilježja žena sa SPP-om. Zaključak: Polimorfizam TNF-alfa -238 mogući je čimbenik podložnosti za SPP, dok polimorfizam TNF-alfa -308 nije čimbenik podložnosti za SPP u žena hrvatske i slovenske populacije.
Abstract (english)
Aim: Tumor Necrosis Factor Alpha (TNF-alpha) is one of the most important pro-inflammatory cytokines, which plays a role in regulating cell differentiation, proliferation and death, as well as inflammation, inborn and adaptive immune responses. It is important in regulation during birth by participating in the remodeling of cervix and fetal membranes by promoting collagen degradation. In our study, we investigated genetic association between TNF-alfa genomic polymorphisms in women and susceptibility to spontaneous preterm birth (SPTB) and we examined their effect on the clinical features of women with SPTB in the Slovenian and Croatian populations. Subjects and Methods: The study was conducted as a case-control study, involving 138 women who had SPTB and 139 women with term births who belonged to the control group. To identify TNF-alpha (-238 G/A or rs361525 and -308 G/A or rs1800629) polymorphisms we used polymerase chain reaction, restriction fragment lenght polymorphism, and gel electrophoresis. Results: The frequency of genotypes and alleles was statistically significantly different in women with SPTB and control group for TNF-alpha -238 polymorphism. Genotypes AG are more frequent in women with SPTB, while genotype GG is more frequent in control group (X2 = 7.32, P = 0.025). Also, in women with SPTB, allele A is more common (X2 = 7.48; P = 0.006). The odds for SPTB is 3, 20 times higher (95% CI= 1,30 – 7,83, P=0,011) in women with AA and AG genotypes than in women with genotype GG. Differences in the frequency of genotypes in TNF -alpha -308 polymorphism among groups of subjects were not statistically significant and no statistically significant association was found between SPTB and any genotype. Conclusion: TNF-alpha -238 polymorphism is a potential susceptibility factor for SPTB, while TNF-alpha -308 polymorphism is not a susceptibility factor to SPTB in the Croatian and Slovene population.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:184:722644
Study programmeTitle: Professional study of Medical and Laboratory Diagnostics (Biomedicine and Healthcare; clinical medical sciences)
Study programme type: professional
Study level: undergraduate
Academic / professional title: stručni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinsko laboratorijske dijagnostike (stručni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
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Created on2018-10-31 11:01:43