The aim of the research in this paper was to find out the attitudes and prejudices of parents towards vaccination of their own children in the Istria. Results: Results were obtained on the basis of 335 respondents, 80.1% of whom were female and mostly with high school education. The respondents are mostly married and mostly belong to the age group of 26-36 years, with a percentage of 46% having two children. An analysis of the attitudes revealed that 90% of parents vaccinate children according to the vaccination calendar, 745 believe vaccinations should be mandatory and 62% of them would vaccinate children and are not legally obliged to vaccinate. The danger of the vaccine is worrying for 43% of parents, with 46% citing side effects as the main reason why they would refuse the vaccine, although 83% of parents had positive experiences with vaccinations. Parents are happy with the information they received about the vaccination, but a modest 35% of the information was collected from doctors and 20% by nurses. The majority of parents 43% understand the importance for the community by keeping their children vaccinated, and a statistically significant difference was found at significance level p <0.05 when asked if they were familiar with the anti-vaccination movement and a relatively high percentage of 36% agreed with the percentage. It has also been reported that parents overwhelmingly agree that vaccination is one of the safest forms of medicine ever developed, that it is important to maintain high vaccination. Parents show concerns about autism linked to vaccination and possible side effects of combination vaccines. Conclusion: the research confirmed the hypotheses: most parents have a positive attitude towards vaccination of their children and have no prejudices against vaccination, and there is a correlation between the demographic and socioeconomic characteristics of parents (age, gender, marital status, educational level, number of children) with attitudes and prejudices about vaccination of their own children.