Breast cancer is the most common malignancy that occurs in women and can also occur in men. It is manifested by the appearance of a new formation in the breast area, and represents a huge public health problem. The aim of the study was to assess whether there is a difference in knowledge between students of full-time and part-time undergraduate professional study of Nursing on breast cancer. The research was conducted among first- and third-year students of full-time and part-time undergraduate professional study of Nursing, and is based on an anonymous online questionnaire. The study involved 100 respondents, the questionnaire consisted of four socio-demographic questions in which age, gender, age and mode of study were examined. The second part of the questionnaire consisted of eleven specific questions on a research topic with multiple choice answers. The obtained results of the survey questionnaire were processed in Excel. For the first hypothesis, based on the t-test value of knowledge about breast cancer, it was shown that the difference of arithmetic means is statistically significant at the significance level of 0.05 (α = 0.05), that is, that there is a statistically significant difference in knowledge between students of the first and the third of full-time and part-time students. For the second hypothesis Based on the t-test of the value of knowledge about the prevention of breast cancer, it was shown that the difference of arithmetic means is not statistically significant at the significance level of 0.05 (α = 0.05), that is, that there is a non-statistically significant difference in knowledge between students of the first and third regular studies and part-time students.