INTRODUCTION: Ophidism is a condition of poisoning by snake toxins. Venomous snakes secrete poison that is toxic to all living organisms because it has a harmful effect on all organic systems. The cardiovascular, nervous, respiratory and urogenital organ systems have the greatest consequences for the human body. Locally, there is rapid development of necrosis and edema, and the general toxic effect of the toxin can lead to acute failure of vital organs. Emergency treatment of a patient in a state of ophidism plays an important role in the patient's survival. OBJECTIVE: The purpose of this research paper is to assess the acquired knowledge of undergraduate Nursing students at the Faculty of Health Studies in Rijeka. RESPONDENTS AND METHODS: The survey was completed by 104 full-time and part-time undergraduate students of Nursing. Students were able to access the survey using a link sent via social networks. The survey consists of 2 parts. The first part of the question has a role in collecting general data, while the second part has a role in testing the acquired knowledge. RESULTS: Based on the obtained results, we can conclude that there is no difference in students' knowledge based on the way of studying and age. It is not possible to test the hypothesis of a difference in knowledge based on completed high school. CONCLUSION: Based on the conducted research and obtained results, we can conclude that students lack knowledge in the field of ophidism. By increasing the number of educations on ophidism, we can directly influence the readiness of students when providing first aid.