Introduction: Sedentary work, such as working in an office or in a vehicle, affects the occurrence of pain in the spine. It is precisely in the mentioned occupations that the spine and surrounding structures are loaded, which is why people begin to have problems related to the lumbar and cervical part of the spine. In addition to prolonged sitting, the occurrence of pain is influenced by the age of the subject, years of work experience, as well as physical activity during free time. Objectives: The main objective of this research paper was to compare the incidence of pain in the cervical and lumbar spine between professional drivers and subjects who spend their professional working day in the office. Subjects and methods: 172 respondents participated in the research, of which 75 were professional drivers and 97 were office workers, with an average age of 40 and 14 years of experience working in the office. The research used a survey questionnaire that contained all questions related to the respondent's job, incidence of pain in a certain part of the spine, and questions related to physical activity. The obtained results were processed in the data processing program R x64 4.0.1 and Microsoft Excel 2010. Data analysis includes descriptive statistics, Pearson's χ2-test of independence, test of proportions and Pearson's correlation coefficient, all at the significance level of p<0.05. Results: Pain in the neck and lumbar part of the spine occurs equally in professional drivers and office workers. Furthermore, pain in the spine occurs more often in people aged 40 or more and in people with 20 or more years of work experience. In addition, pain in the spine occurs equally in people who perform physical activity more than 2 times a week for a minimum of 30 minutes, as well as in people who perform physical activity less than 2 times a week. Likewise, there is a medium-strength correlation between years of service and time spent on sick leave. Conclusion: Pain in the spine occurs equally in both groups of subjects, which leads to the conclusion that the sedentary way of working, more specifically the job of a professional driver and office worker, has a significant impact on the health of the spine. It can also be concluded that older people have more spine problems than younger people.