Infertility is a term that denotes an unsuccessful achievement of clinical pregnancy after a minimum of 12 months of regular and unprotected sexual intercourse between a man and a woman. People struggling with infertility treatment often show symptoms of stress, anxiety and depression. In about 85% of cases, the cause of infertility is known and therefore can be adequately treated. Ways of treating infertility in the Republic of Croatia are regulated by the Law on Medically Assisted Fertilization. The role of nurse/technician and midwife is important during health care in education and support during the physical and psychological preparation of couples for the treatment of infertility. The aim of this research paper is to examine the knowledge of full-time and part-time students of the undergraduate study of Nursing and undergraduate study of Midwifery about the treatment of infertility. The specific goals are to see if there are any differences in knowledge about infertility treatment between undergraduate nursing students and midwifery undergraduate students, as well as to see if there are any differences in knowledge about infertility treatment between first and the third year nursing undergraduate students. The total number of students who joined the survey is 106. The survey questionnaire consisted of 6 socio-demographic questions and 15 questions related to the knowledge of nursing students and midwifery students about the treatment of infertility. The resulting survey questionnaire data were statistically processed in Microsoft Office Excel.