We distinguish between monotheistic and polytheistic religions and numerous cultural differences. The main cause of this is global migration and the emergence of different beliefs and traditions around the world. In the healthcare system, medical professionals often encounter patients who are of a different religion or culture than themselves. Nurses/technicians should know the specifics of different cultures and religions and also possess excellent communication skills in order to achieve a positive and trusting relationship with the patient and to provide the best healthcare. The aim of the research is to examine the attitudes of nursing students of the Faculty of Health Studies in Rijeka about the way of working with patients of different cultures and religions during their professional practice. We also want to find out whether full-time and part-time nursing students are more or less sensitive to the different cultural and religious needs of patients. The research was conducted during March and April at the Faculty of Health Studies of the University of Rijeka. 102 undergraduate nursing students participated in the research. The data for this research was collected based on a questionnaire created in Google forms. The questions in the questionnaire are divided into 2 parts. The research results showed that the first and second hypotheses were partially accepted, while the third hypothesis was fully accepted. The first hypothesis was partially accepted because there is a statistically significant difference in the attitudes of first-year nursing students of the Faculty of Health Studies in Rijeka about working with patients of different religions and cultures, observed in only two statements, but other data show that regular students are still more tolerant. The second hypothesis is also partially accepted because in most questions the difference in answers is negligible and the positive attitudes of regular second-year students prevail. The third hypothesis was fully accepted and proves that full-time 3rd-year nursing students and part-time 3rd-year nursing students of the Faculty of Health Studies in Rijeka have positive attitudes about working with patients of different religions and cultures.