INTRODUCTION AND OBJECTIVE OF THE RESEARCH: The HPV virus poses a considerable threat to the current population as it is presently the most prevalent sexually transmitted disease. In addition to the symptoms it causes, it represents a great risk for the development of cervical cancer, which is among the most prevalent cancers today. Early education and preventive measures can substantially decrease the occurrence of cervical cancer and other symptoms caused by the HPV virus. For this reason, the goal was to examine the knowledge and attitudes of students of the Faculty of Health Studies in Rijeka about HPV infection and vaccination against HPV. MATERIALS AND METHODS: The study involved full-time undergraduate students enrolled in the Faculty of Health Studies in Rijeka. An electronic questionnaire was utilized, specifically designed on Google Forms solely for this research purpose. 120 students took part in the research, of which students of Nursing (43.3%), Physiotherapy (24.2%), Radiological Technology (18.3%) and Midwifery (14.25%). RESULTS: The results show that the vaccination rate of students at the Faculty of Health Studies is (44.3%). Students across various fields of study displayed equally positive attitudes toward HPV vaccination, with no significant differences observed in between different studies. It was confirmed that nursing students have better comprehension about HPV infection and the HPV vaccine, compared to physiotherapy and radiological technology students. In addition to nursing students, midwifery students showed better knowledge compared to physiotherapy students. Also, it was confirmed that vaccinated students have more knowledge about Human papillomavirus and vaccination, compared to unvaccinated students. CONCLUSIONS: Nursing students do not have better knowledge than midwifery students, but they have better knowledge than physiotherapy and radiological technology students. All students hold equally positive attitudes towards vaccination, and vaccinated students have better knowledge compared to non-vaccinated students. These findings underscore the necessity for population education that empowers individuals to make informed decisions regarding their health. Moreover, additional education for healthcare professionals regarding HPV and HPV vaccine is crucial, as they serve as primary educators and advocates for community health.