Introduction: Human papillomavirus (HPV) is a widespread viral sexually transmitted disease from which no age group is exempt. It represents a major public health problem. Public health efforts are aimed at preventing the occurrence and spread of HPV infections among the population through action in the school environment on the target group of adolescents, education and information for parents, vaccination and promotion of regular gynecological examinations of women and the use of protective equipment during sexual intercourse. With the progress of medicine and the development of new vaccines, it is important to educate the population in order to become familiar with the importance of vaccines and prevention. Objective: To establish the attitudes of the general population about HPV infection and vaccination and the number of vaccinated persons by gender in Zadar County. To determine the reasons for not vaccinating against HPV infection among respondents in Zadar County. Methods: The one-month study included 100 respondents in Zadar County between the ages of 18 and 25. The online survey consisted of 20 questions, based on which the answers were analyzed by gender and age. Results: The majority of respondents in Zadar County have positive attitudes about vaccination against HPV infection. More than 50% of respondents in Zadar County were not vaccinated against HPV infection. Vaccination against HPV infection is not higher in female respondents compared to male respondents. Most respondents in Zadar County do not have enough knowledge about HPV infection and vaccination against HPV infection. Conclusion: All research participants, regardless of age or gender, have positive attitudes about vaccination against HPV infection, but only 50% were vaccinated. Vaccination is equal for men and women. In Zadar County, education is needed regarding HPV infection and vaccination against HPV infection.