Aim: To determine the frequency of malignant lesions of the neck region detected by cytological analysis after aspiration with a thin needle under ultrasound control. Then analyze the localization of suspicious lesions within the neck region, and determine the distribution of fine needle aspiration with regard to the gender and age of the patients. Subjects: This retrospective study included patients who, in the period from January 1, 2022 to December 31, 2022, underwent fine-needle cytological aspiration under ultrasound control of lesions in the region of the neck, inguinum, and axilla, at KBC Rijeka at the Clinical Institute for diagnostic and interventional radiology. From the electronic medical records (obtained from IBIS), we recorded demographic data (age, sex) and the results of cytological analyzes of fine needle aspiration. Results: In a period of one year, a total of 180 thin needle aspirations were performed under ultrasound control, of which 45 (25%) were performed in men and 135 (75%) in women. The average age of the subjects was 61 ± 15 years. The youngest respondent was 20 years old, and the oldest was 86 years old. Most fine needle aspiration was done in the neck region, 136 of them (75.56%). In the largest number of aspirates performed, the cytological findings corresponded to lymphatic tissue without malignant elements (45; 25%), while the rarest cytological findings were plasmacytoma and pleomorphic adenoma. Malignant lesions are equally represented in men and women (P=0.59). Aspirations with a fine needle of the thymus are significantly more frequent than other aspirations within the neck region (P< 0.001). Conclusion: Cytological fine needle aspiration (FNA) under ultrasound control is a safe and effective method for detecting and differentiating suspicious lesions in the neck region, as well as in the axilla and inguinal regions.