The primary objective of the study was to examine whether there are differences between blood donors and non-blood donors in latent profiles of stable prosocial tendencies. A cross-sectional survey was conducted using an online questionnaire in the population of citizens of Samobor who were registered in the Register of Blood Donors of the Red Cross Samobor and in the population of parents of students in one kindergarten and one high school. The survey was conducted from May 14 to June 3, 2020. The target population consists of voluntary blood donors permanently residing in Samobor during 2019. The main outcome was the difference in latent profiles of prosocial tendencies measured using the “Prosocial Tendency Measure”. The primary independent variable was blood donor or non-blood donor status. The samples of 118 donors and 91 non-donors were selected. Five different profiles of prosocial tendencies were found in the blood donor population. An equal number of different profiles of prosocial tendencies were also found in the non-donor population, but the nature and frequencies of these profiles were most often different from those in the donor population. Profiles of prosocial tendencies recognized in both the donor population and the non-donor population are called: "prosociality exclusively for the public", "complex emotional prosocial tendency with moderate altruism", "pure altruism". Two profiles were primarily characteristic of the donor population: "altruism" and "prosociality for the public with personal and social motives", and two only for the non-donor population: "mild compliant" and "emotional prosocial tendencies". Thus, the research confirmed the hypothesis of differences in the latent profiles of prosocial tendencies between blood donors and non-donors.