The research was conducted on 60 students of the Faculty of Health Studies and 64 students of the Faculty of Civil Engineering, University of Rijeka. The aim of the research was to determine the knowledge and attitudes of students at the University of Rijeka about vaccination and their dependence on the type of studies, sources of information, years of experience and parenthood. The research was conducted through an online questionnaire consisting of 4 parts. In the first part, the respondents were asked about general data, in the second part their knowledge was examined, and in the third part attitudes about vaccination was examined. The last part of the questionnaire contained questions about sources of vaccination information. The results showed that students of the Faculty of Health Studies have more knowledge about vaccination, but that their attitudes about it are not statistically significantly more positive than the attitudes of students at the Faculty of Civil Engineering. The results showed that students who stated that they most often get vaccination information from reliable sources have more knowledge than those who most often get information from unreliable sources. However, attitudes about vaccination are not statistically significantly more positive in students who use reliable sources compared to those who use unreliable sources. Students who work a larger number of years have more knowledge about vaccination than those who work a smaller number of years, however, even in these two groups the attitudes do not differ statistically significantly. Also, the differences between students who are parents and those who are not in attitudes and knowledge about vaccination did not prove to be statistically significant. It is important to implement vaccination education in biomedical education from the earliest levels, but also to educate students of other specialties about the same through public health programs.