Diseases of the cardiovascular system are the leading cause of death in the Republic of Croatia as well as in most countries around the world. The etiology of these diseases is multifactorial and most of the risk factors that cause cardiovascular disease are considered to be preventable. The aim of this study is to analyze the eating habits of the working population considerating the risk factors for cardiovascular diseases that are common in our population and to investigate the relationship of risk factors in relation to blood biomarkers and clinical manifestations, also to identify the effects of various risk factors. The research was conducted at the Medico Special Hospital in Pula. The subjects who participated in this study were working-able patients of the named hospital who underwent regular systematic examination. Data on eating habits, physical activity and general health status were obtained using a questionnaire and data from laboratory biomarkers, anthropometric measures and clinical data from the subjects were collected. There were statistically significantly more obese men compared to women (p <0.001). Women had a statistically significantly lower waist circumference than men (p <0.001) and higher HDL (p = 0.0002), while men had significantly higher triglyceride and glucose concentrations (p = 0.0006; p = 0.0012). Men had a statistically significantly higher CMI than women (p = 0.0005). Subjects who were very active had the best MDS score (p <0.001) and subjects with a better MDS score had a smaller waist circumference and BMI. This study showed that male subjects had a higher risk of developing a cardiovascular disease and that physical activity and dietary quality affected the body composition and weight of subjects which are important factors in the development of chronic diseases.