Introduction: Emergency medicine includes daily encounters with life-threatening individuals, exposure to high levels of stress and emotional suffering, inadequate working conditions, exposure to infections, contaminated biological material and chemicals, hard-to-reach intervention sites and frequent encounters with violence. These factors may be responsible for the occurrence of psychological, physical and emotional stress in emergency workers. High levels of stress and working conditions negatively affect the quality of life of health professionals because the difficulties they face can have negative effects on their physical and mental health and social functioning. Objective: The main objective of this research is to analyze the quality of life of health professionals of the Institute of Emergency Medicine of Karlovac County in relation to socio-demographic and work characteristics of respondents Methods: The research was conducted on a sample of 70 respondents who are employed in the Institute of Emergency Medicine of Karlovac County. The WHOQOL ‐ BREF Quality of Life Questionnaire was used to examine the level of quality of life Results: The quality of life among health professionals in the Institute of Emergency Medicine of Karlovac County is satisfactory. Female respondents were statistically significantly more dissatisfied with psychological health than male respondents. A statistically significant difference was found in the field of environment in which respondents in the age group of 36 to 45 years achieved a statistically significantly lower result than respondents in other age groups. Doctors do not show statistically significantly higher results in any part of the questionnaire than nurses / technicians, while in the field of mental health nurses show a higher level of quality of life compared to doctors. Conclusion: The emergency medical service is one of the most stressful work environments due to heavy workloads, patient conditions, time constraints, rapid decision-making in critical situations and lack of human and technical resources. Working in an ambulance service can therefore lead to a poor quality of life, which in turn can lead to a poor quality of service for patients.