INTRODUCTION: Basic life support methods and the use of an automatic external defibrillator greatly reduce the percentage of fatal and adverse outcomes of cardiac and/or respiratory arrest. Through their education, the nurse and technician are trained in the ABCDE approach to the patient, as well as in the scale for assessing the state of consciousness, in addition, they know how to recognize signs of suffocation, cardiac and respiratory arrest, and help methods. OBJECTIVE: The aim of the research is to determine whether the nurses and technicians of the Rijeka Clinical Hospital Center have sufficient knowledge about basic life support. The specific objectives are to determine the difference in the knowledge of nurses and technicians regarding the level of education, and the level of knowledge of employees in the departments of anesthesiology, intensive care medicine and unified emergency hospital admission compared to other departments. RESPONDENTS AND METHODS: A voluntary, anonymous survey was conducted using a two-part survey questionnaire, where the first part examines sociodemographic data, and the second part examines knowledge through 26 questions, and 226 respondents participated. RESULTS: Results were presented using descriptive statistics, arithmetic mean and standard deviation, while hypotheses were tested using T-test for one independent measurement, T-test for 2 independent measurements, and ANOVA test with LSD post hoc test. This research confirmed that respondents have satisfactory knowledge of basic life support, respondents with a higher level of education have more knowledge than those with secondary education, and nurses and technicians employed in the departments of anesthesiology, intensive care medicine and unified emergency hospital admission have more knowledge than of other respondents employed in other departments. CONCLUSION: These results confirm the knowledge of nurses and technicians about basic life support, however, the least correct answers occur in the area of ​​life support when it comes to children, which requires additional education in that specific area. The knowledge and skills acquired during schooling and work need to be renewed, and additional training, practical workshops and the like should be carried out in order to be able to react adequately, faster and better in a real situation.