Introduction: Multiple sclerosis is a chronic, autoimmune, inflammatory, neurodegenerative disease of the central nervous system. The exact cause of the disease is unknown, but it is assumed to be a result of the interaction of genetic and environmental factors. It usually occurs between the ages of 20 and 50 and is about 2-3 times more common in women. It is manifested by the appearance of numerous symptoms that can result in disability and reduced quality of life. In order to maintain functional ability, strength, mobility, and quality of life, it is essential to conduct exercises continuously. Aim: The aim of this study was to determine the effect of exercise on quality of sleep and quality of everyday life in patients with MS. Participants and methods: 24 examinees with MS and 25 healthy subjects participated in the study. Two groups of them practiced, while two groups did not exercise. The study was conducted in the premises of the Multiple Sclerosis Association of Primorsko-goranska County in Rijeka, continuously over a period of 2 months, 2 times a week for 60 minutes. The exercise program included breathing exercises, stretching exercises, exercises to increase the volume of movement and muscle strength, and balance exercises and coordination exercises under the supervision of a physiotherapist. Several questionnaires were used to assess the quality of sleep and everyday life of the respondents, namely PSQI, ISI, SF-36 and a subjective questionnaire on the impact of MS exercise on health. Results: Based on the results, there was a difference in the values before and after the exercise program. In the MS patients who performed the exercise, there was improvement in sleep and reduction of insomnia. Also, there was an increase in the quality of life after exercise in all aspects of life, and in the physical limitation that became less pronounced. Most respondents felt better upon completing the exercise program and were motivated to continue exercising at home. Conclusion: Accordingly, it can be concluded that the exercise program has had a positive impact on the quality of sleep and everyday life in patients with MS.