Introduction: Nursing is a profession that is considered one of the most stressful professions in the world. The challenge for the healthcare system and its participants is the COVID-19 disease, which the World Health Organization declared a pandemic on March 11 th , 2020. Ignorance of the disease at the beginning of the pandemic, uncertainty of the situation, fear of the unknown, working in protective equipment that makes many feel discomfort and anxiety require healthcare workers to adapt to work in new conditions. Objective:To examine the sources and level of stress among nurses and technicians at Karlovac General Hospital who worked with COVID positive patients. Subjects and methods: 71 subjects, nurses and technicians of Karlovac General Hospital, of all levels of education, who participated in work with COVID positive patients in the period from March 2020 to March 2022, participated in the research. Data were collected by completing a survey consisting of 34 questions, collected through a google form. The data has been processed in the IBM SPSS, Statistic 23.0 program package (SPSS, Chicago, IL, USA). Results: The results obtained from the conducted research show that the respondents expressed a high level of stress on all tested sources of stress. Working in protective equipment and with COVID-positive patients causes a high level of stress for nurses and technicians at OB Karlovac. The research shows that no statistically significant differences were found in the experienced stress between respondents who worked at one workplace and those who worked at several workplaces during one shift. Also, no statistically significant difference was found between the group of respondents who worked for less than 6 months and the group who worked for more than 6 months in the COVID department. The connection between the stress caused by the absence of a doctor in the emergency intervention of the care of a COVID-positive patient and the total stress has been proven. Conclusion: The results obtained from the conducted research are worrisome because the subjects expressed a high level of stress. The uncertainty of the end of the pandemic is also worrying. This research was conducted on a small convenient sample, so further research into the experiences of working in the field of caring for COVID positive patients is very necessary.