Introduction: One of the basic prerequisites for optimal image recording on mammography is the compression force that positions the breast in a stationary position. Depending on the applied compression force, the degree of discomfort felt by the patient during the procedure varies. The density of the breast tissue is one of the important parameters that affect the compression force, and it is divided into four categories, depending on the proportion of glandular tissue. Objective: The objective of this research is to investigate the influence of breast tissue density, the age of the subjects and the compression force during mammography on the perception of pain during the performance of this procedure. Materials and methods: 99 female subjects participated in this research in the period from May 22 to June 30, 2023 at the Department of Abdominal Radiology and Breast Radiology of the Clinical Institute for Diagnostic and Interventional Radiology of the Clinical Hospital Center in Rijeka. The research included conducting a survey that examined the level of pain using a numerical scale (from 0 to 10 points), immediately after the mammography procedure. The second part of the research involved collecting data on breast tissue density and applied compression force from hospital databases. Statistical analysis of data was calculated using Kolmogorov-Smirnov, ANOVA, χ2 and Mann-Whitney U test, all at the p<0.05 level of statistical significance. The Statistica 14.0.0.15 program (TIBCO Software Inc.) was used for data processing. Results: The most common category of breast tissue density among the test subjects was category B, while only one case of density category D was recorded. The mean pain level was 5. The mean compression force on the right breast in the CC projection was 98,80 N, while the mean level of compression force on the left breast was 97,62 N. The mean compression force on the left breast in the MLO projection was 118,86 N, and on the right breast 114,81 N. Conclusion: The mean age of the subjects, the representation of individual ACR categories and the applied compression force are in accordance with other research and guidelines, as well as the results of hypothesis testing. The only deviation is in the mean level of reported pain, which is higher compared to the results of other studies.