INTRODUCTION: Prostate cancer is the most common visceral cancer and the second most common cause of death in men. In the diagnostic procedure of prostate cancer, the most common complications that arise after a prostate biopsy are hematuria, uroinfection and urinary retention. About 200 prostate biopsies are performed annually at Karlovac General Hospital. RESEARCH GOAL: The aim of this study was to analyze whether the age and presence of comorbidities of patients have an impact on the greater incidence of complications after prostate biopsy, as well as what are the most common early and late complications, and comorbidities among subjects. RESPONDENTS AND METHODS: The sample consisted of 107 male subjects diagnosed with suspected prostate cancer between 50 and 85 years, who had a prostate biopsy performed at the Karlovac General Hospital at the Department of Urology in the period from 01.04.2023. to 31.07.2023. Patients were given an anonymous questionnaire with questions about early and late complications after prostate biopsy, and the presence of comorbidities. The difference between the proportions was tested by a chi-square test. The statistical significanceis set to p≤0.05. RESULTS: Out of the total number of subjects (N =107), early complications like fever after prostate biopsy reported 15 (14.02%). Chills or shaking was reported by 6 subjects (5.61%). Among them, 8 (7.47%) reported to the hospital, and 3 (2.80%) were hospitalized. The most reported early complication of prostate biopsy is blood in the urine (32.12%). Late complications after prostate biopsy in the form of urination problems were reported by 47 subjects (43.93%). The most reported late complication of prostate biopsy is difficulty urinating (31.67%). The most reported comorbidity among subjects is cardiovascular disease (N=53;45.69%). Comorbidities are more common in the older age group (N=72;62.07%). CONCLUSION: Although prostate biopsy continues to be the gold standard in diagnosing and monitoring prostate cancer, patient screening should be considered considering individual characteristics and risk factors, especially comorbidities.